P-246: Gene Variation of TLR4 in Patients with Endometriosis

Background: Endometriosis, defined as the presence of endometrial tissue outside of the uterus, is an estrogen-dependent chronic inflammatory condition associated with degrees of pelvic pain and infertility. Toll-like receptors play a key role in immune response, by regulating inflammatory reactions and activating adaptive immune response to eliminate infectious pathogens and cancer debris. Polymorphisms in TLR4 have been shown to be associated with increased susceptibility to diseases such as inflammation and cancer. Ectodomain of TLR4 protein consists of 21 leucine-rich repeats (LRRs) that are crucial for its dimerization and signaling. The aim of this study was to determine gene variations of TLR4 in patients with endometriosis. Materials and Methods: In this case-control study 65 blood samples were recruited from endometriosis patients referring to Royan Institute during 2012-13, who have been confirmed by laparoscopic surgery and 45 fertile women as control group that had no history of inflammatory disorders or using any related drugs. Ethical approval forms were obtained prior to the samples collection. DNA was extracted by kit, special primers were designed, the LRR coding region was amplified by PCR and products were analyzed by sequencing. Results: One out of 65 patients had a heterozygote single nucleotide polymorphism (SNP) in (rs4986790) Asp299Gly. Conclusion: According to our results we suggest that ASP299GLY polymorphism be involved in pathogenesis of endometriosis although more studies are required in this regard.